Wednesday 24
The specific sequence- and epigenetic state-dependent folding of chromosomes

› 9:00 - 9:45 (45min)
Nucleosome-positioning & Long-range contacts at Topological boundaries
Olivier Cuvier  1@  
1 : Laboratoire de biologie moléculaire eucaryote du CNRS  (LBME)  -  Website
CNRS : UMR5099, Université Paul Sabatier (UPS) - Toulouse III
Bât IBCG 118 Route de Narbonne 31062 TOULOUSE CEDEX 4 -  France

We shall discuss our recently published data together with unpublished stuffs highlighting a role of insulator proteins and their co-factors in functional long-range contacts. Our systematic analyses revealed hidden information in ChIP-Seq that may be used to predict long-range contacts in chromosomes. Further mathematical 'aggregation' of genome-wide Hi-C data confirms the relevance of such predicted long-range contact maps, defining a potent tool for determining contacts at a high resolution (< 500 bp). Such approach is currently being adapted for a broad usage by the research community of this tool as a package.

We will also present our published and unpublished results highlighting a role of the Histone-methyl transferase Mes4/NSD and dHypb/Set2 in transcription-dependent nucleosome positioning that controls progression of RNA polymerase through gene bodies. Our data further identifies Set2 as a key regulator of nucleosome positioning, involving the recruitment of chromatin remodelers to gene bodies.



Chromatin immunoprecipitation indirect peaks highlight long-range interactions of insulator proteins and Pol II pausing. Liang J, Lacroix L, Gamot A, Cuddapah S, Queille S, Lhoumaud P, Lepetit P, Martin PG, Vogelmann J, Court F, Hennion M, Micas G, Urbach S, Bouchez O, Nöllmann M, Zhao K, Emberly E, Cuvier O. Mol Cell. (RESOURCE Article). 2014;53:672-81.

Insulators recruit histone methyltransferase dMes4 to regulate chromatin of flanking genes. Lhoumaud P, Hennion M, Gamot A, Cuddapah S, Queille S, Liang J, Micas G, Morillon P, Urbach S, Bouchez O, Severac D, Emberly E, Zhao K, Cuvier O. EMBO J. 2014;33:1599-613.

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